linka 3840 (tel. 220 443 840)
linka 5173 (tel. 220 445 173)
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Vzdělání a kvalifikace
- 2014
- Krátkodobá stáž v laboratoři Dr. Delphine Muriaux, CPBS, CMRS, Montpellier, Francie
- 2010-
- vědecký pracovník v laboratoři NMR spektroskopie, VŠCHT Praha
- 2007–2013
- postgraduální studium v oboru Biochemie, Fakulta potravinářské a biochemické technologie, VŠCHT Praha (téma: Studie interakce matrixového proteinu Masonova-Pfizerova opičího viru s cytoplasmatickou membránou)
- 2002–2007
- magisterské studium v oboru Obecná a Aplikovaná Biochemie, Fakulta potravinářské a biochemické technologie, VŠCHT Praha (téma: Studium struktury mutantu T41I/T78I matrixového proteinu Mason-Pfizerova opičího viru)
Oblasti výzkumu
Virologie, studium struktury a funkce proteinů pomocí NMR spektroskopie.
Vybrané publikace
Vlach J., Srb P., Prchal J., Grocký M., Lang J., Ruml T., Hrabal R. (2009) Nonmyristoylated Matrix Protein from the Mason-Pfizer Monkey Virus Forms Oligomers. J. Mol. Biol., 390(5), 967–980. doi:10.1016/j.jmb.2009.05.063
Srb P., Vlach J., Prchal J., Grocký M., Ruml T., Lang J., Hrabal R. (2011) Oligomerization of a retroviral matrix protein is facilitated by backbone flexibility on nanosecond time scale. J. Phys. Chem. B, 115(11), 2634-2644.doi: 10.1021/jp110420m
Prchal J., Junková P., Strmisková M., Lipov J., Hynek R., Ruml T., Hrabal R. (2011) Expression and purification of myristoylated matrix protein of Mason-Pfizer monkey virus for NMR and MS measurements. Protein Expr. Purif., 79(1), 122-127. doi: 10.1016/j.pep.2011.05.010
Prchal, J., Srb, P., Hunter, E., Ruml, T., Hrabal, R. (2012)The Structure of Myristoylated Mason-Pfizer Monkey Virus Matrix Protein and the Role of Phosphatidylinositol-(4,5)-bisphosphate in its Membrane Binding. J.Mol.Biol., 423(3), 427-438. doi:10.1016/j.jmb.2012.07.021
Junkova, P., Vermachova, M., Prchal, J., Kuckova, S., Hrabal, R., Hynek, R. (2013) Improved Approach for the Labeling of Argininne, Glutamic, and Aspartic Acid Side Chains in Proteins Using Chromatographic Techniques. J. Liq. Chromatogr. Relat. Technol., 36(9), 1221-1230. doi:10.1080/10826076.2012.685918
Rozbeský, D., Krejzová, J., Křenek, K., Prchal, J., Hrabal, R., Kožíšek, M., Weignerová, L., Fiore, M., Dumy, P., Křen, V., Renaudet, O. (2014) Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides. Int. J. Mol. Sci., 15(1), 1271-1283. doi:10.3390/ijms15011271
Prchal, J., Kroupa, T., Ruml, T., Hrabal, R. (2014) Interaction of Mason-Pfizer monkey virus matrix protein with plasma membrane. Front. Microbiol. 4(423), doi: 10.3389/fmicb.2013.00423
Kroupa, T., Prchal, J., Doležal, M., Ruml, T., & Hrabal, R. (2014). Cost-effective method for the preparation of uniformly labeled myristoylated proteins for NMR measurements. Protein Expr. Purif., 99, 6-9. doi:10.1016/j.pep.2014.03.005
Junková, P., Prchal, J., Spiwok, V., Pleskot, R., Kadlec, J., Krásný, L., Hynek, R., Hrabal, R. and Ruml, T. (2016), Molecular aspects of the interaction between Mason—Pfizer monkey virus matrix protein and artificial phospholipid membrane. Proteins, 84: 1717–1727. doi:10.1002/prot.25156
Kerviel A, Dash S, Moncorgé O, Panthu B, Prchal J, Décimo D, et al. (2016) Involvement of an Arginine Triplet in M1 Matrix Protein Interaction with Membranes and in M1 Recruitment into Virus-Like Particles of the Influenza A(H1N1)pdm09 Virus. PLoS ONE 11(11): e0165421. doi:10.1371/journal.pone.0165421
Kroupa T., Langerová H., Doležal M., Prchal J., Spiwok V., Hunter E., Rumlová M., Ruml T., Hrabal R. (2016), Membrane Interactions of the Mason-Pfizer Monkey Virus Matrix Protein and Its Budding Deficient Mutants. J.Mol.Biol. 428(23):4708-4722. doi: 10.1016/j.jmb.2016.10.010.
JUNKOVA, P.; PLESKOT, R.; PRCHAL, J.; SYS, J. et al. Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV (vol 295, pg 8819, 2020). Journal of Biological Chemistry, 295, n. 31, p. 10869-10869, Jul 2020. DOI: 10.1074/JBC.AAC120.015073
PRCHAL, J.; SYS, J.; JUNKOVA, P.; LIPOV, J. et al. Interaction Interface of Mason-Pfizer Monkey Virus Matrix and Envelope Proteins. Journal of Virology, 94, n. 20, Oct 2020. DOI:10.1128/JVI.01146-20