Mass Spectrometry (MS) laboratory, a part of university central laboratories, provides analytical service in mass spectrometry. Mass spectra of organic substances and their mixtures are measured. Separation techniques of gas chromatography (GC) and reverse-phase liquid chromatography (RP-HPLC) are used.
Available ionization techniques
GC/MS, direct insertion probe, batch inlet:
- Electron Ionization (EI+ 70 eV)
- Methan Chemical Ionization (CI)
LC/MS, FIA (Flow Injection Analysis), RP-HPLC
- Elektrospray ionization (ESI) – positive, negative mode
- Atmospheric Pressure Chemical Ionization(APCI) - positive, negative mode
Mass spectra acquisition
- low (unit) rosolution
- high resolution – mass accuracy better than 5 ppm (2 ppm with a lock-mass)
- acqusition of MS/MS spectra available (ESI, APCI MSn available)
Mass spectra m/z range
- EI + 70 eV, CI: limited by samples stability and possibility of vaporization (usually up to 1000 Da)
- ESI, APCI: up to 4000 Da (valid for single charged ions)
Analyses
- molecular weight, elemental composition confirmation
- organic mixtures analyses (GC/MS)
- organic mixtures analyses (RP HPLC/MS, RP HPLC/UV-DAD) (consultation required)
- quantitative analyses, pollutants screening in complex matrices (consultation required)
- structure characterization – MS/MS experiments, EI+ 70eV
- GC/MS a LC/MS method development (consultation required)
Samples pre-treatment (consultation required)
- SPME, SPE, MEPS etc.
- thermal desorption (TD) – GC/MS
VOC (Volatile Organic Compounds) analyses in atmosphere or gases , emmited from materials (consultation required)
- Tedlar bag sampling
- thermal desorption tubes (e.g.TENAX) sampling
- solvent desorption tubes (e.g. ORBO, DNPH) sampling
- SUMMA cannister sampling
Analyses results
Mass spectra and additional information are provided in the form of pdf files via e-mail, raw files data are provided on a request. Mass spectra can be provided as weel as Excel or Word files including their metadata and list form.